The Immunoglobulin G2a (IgG2a) BioAssay™ ELISA Kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of IgG2a in rat serum, plasma and other biological fluids.
Detection Range:246.91-20,0000ng/ml
Sensitivity:96.3ng/ml
Intra-Assay CV:<10%
Inter-Assay CV:<12%
Kit Components:*152701A: Microtiter Strips, 1x96 wells, Pre-coated, ready to use*152701B: Standard, 2x1 vial152701C: Standard Diluent, 1x20ml*152701D: Detection Reagent A (green), 1x120ul*152701E: Detection Reagent B (red), 1x120ul152701F: Assay Diluent A, 1x12ml152701G: Assay Diluent B, 1x12ml152701H: TMB Substrate, 1x9ml*152701J: Positive Control, 1x1vial, xxng/ml (Lyophilized)152701K: Stop Solution, 1x6ml152701L: Wash Buffer, 30X, 1x20ml
Precaution:The Stop Solution (152701K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Storage and Stability:Store *152701A, *152701B, *152701D, *152701E and *152701J at -20°C. After reconstitution of *152701J, store at 4°C and use within 5 days. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
Assay Procedure Summary:1. Prepare all reagents, samples and standards
2. Add 50ul standard or sample to each well
3. Add 50ul prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C
6. Aspirate and wash 5 times
7. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37°C
8. Add 50ul Stop Solution. Read at 450nm immediately.
