The Human Transferrin (TRF) ELISA Kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of TRF in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Precaution:The Stop Solution (152677K) included for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Notes on 152677J: Positive Control:Intended Use:The control vials are from a human serum sample known to contain Transferrin. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly.
Characteristics:The control vials contain mouse Transferrin in human serum (lyophilized powder). Note: The human based serum used in this product are screened for HIV, Hepatitis A, B, C, HTLV and Syphilis.
Expected Values:2.71ng/ml
Usage:Dissolve with 150ul standard dilutent and let stand for 10 minutes. If powder is partly insoluble, shake gently until fully dissolved. Mix gently before use. In the experiment, add 100ul to an appropriate well of the plate and test its concentration along with standards and your specimens. Note: Upon receipt store Positive Control vial at -20°C. After reconstitution, store Positive Control vial at 4°C and use within 5 days.
Storage and Stability:Store *152677A, *152677B, *152677D, *152677E and *152677J at -20°C. After reconstitution of *152677J, store at 4°C and use within 5 days. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing
Assay Procedure Summary:1. Prepare all reagents, samples and standards
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C
6. Aspirate and wash 5 times
7. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37°C
8. Add 50ul Stop Solution. Read at 450nm immediately.
仕様
Size：96Tests
Source Antigen：Human
Specificity：This assay has high sensitivity and excellent specificity for detection of High Sensitive Transferrin (TRF). No significant cross-reactivity or interference between High Sensitive Transferrin (TRF) and analogues was observed.
