The Human Heat Shock Protein 90 (HSP90) ELISA Kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of HSP90 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.
Detection Range:3.125-200ng/ml
Sensitivity:<1.28ng/ml
Precision:Intra-Assay: CV<10%Inter-Assay: CV<12%
Test Principle:The microtiter plate provided in this kit has been pre-coated with an antibody specific to HSP90. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to HSP90. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HSP90, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HSP90 in the sample is then determined by comparing the O.D. of the sample to the standard curve.
Storage and Stability:Store *152642A, *152642B, *152642D and *152642E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
仕様
Size：96Tests
Source Antigen：Human
Specificity：This assay has high sensitivity and excellent specificity for detection of Heat Shock Protein 90 (HSP90). No significant cross-reactivity or interference between Heat Shock Protein 90 (HSP90) and analogues was observed.
