Intended Use:The Mouse Fibrinopeptide A (FPA) ELISA Kit is a competitive inhibition immunoassay for the in vitro quantitative measurement of FPA in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.
Detection Range:370.4-30,000pg/ml
Sensitivity:137.3pg/ml
Intra-Assay CV:<10%
Inter-Assay CV:<12%
Kit Components:*152564A: Microtiter Plate, 1x96 wells*152564B: Standard, 2x1 vial 152564C: Standard Diluent, 1x20ml*152564D: Detection Reagent A (green), 1x1vial *152564E: Detection Reagent B (red), 1x120ul 152564F: Assay Diluent A, 1x12ml152564G: Assay Diluent B, 1x12ml152564H: TMB Substrate, 1x9ml152564K: Stop Solution, 1x6ml152564L: Wash Buffer, 30X, 1x20ml152564M: Reagent Diluent for 152564D, 1x300ul
Precaution:The Stop Solution (152564K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Storage and Stability:Store *152564A, *152564B, *152564D and *152564E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
Assay Procedure Summary:1. Prepare all reagents, samples and standards
2. Add 50ul standard or sample to each well, then add 50ul prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
3. Aspirate and wash 3 times
4. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C
5. Aspirate and wash 5 times
6. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37°C
7. Add 50ul Stop Solution. Read absorbance at 450nm immediately.
