Kit Components:*152477A: Microtiter Plate, 1x48 wells or 1x96 wells, Pre-coated, ready to use*152477B: Standard, 2x1vial 152477C: Standard Diluent, 1x10ml or 1x20ml*152477D: Detection Reagent A (green), 1x1vial*152477E: Detection Reagent B (red), 1x60ul or 1x120ul 152477F: Assay Diluent A, 1x6ml or 1x12ml152477G: Assay Diluent B, 1x6ml or 1x12ml152477H: TMB Substrate, 1x4.5ml or 1x9ml*1524774J: Positive Control, 628.3ng/ml, 1x1vial152477K: Stop Solution, 1x3ml or 1x6ml152477L: Wash Buffer, 30X, 1x10ml or 1x20ml152477M: Reagent Diluent for 152477D, 1x300ul
Precaution:The Stop Solution (152477K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Storage and Stability:Store *152477A, *152477B, *152477D, *152477E and *152477J at -20°C. After reconstitution of *152477J, store at 4°C and use within 5 days. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
Notes on 152477J: Positive Control:Intended Use:The positive control is from rabbit plasma sample known to contain the target protein. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly.
Characteristics:The control vial contains rabbit D2D in rabbit plasma (lyophilized powder). Note: The rabbit based serum used in this product are from healthy rat of SPF standard (Specific Pathogen-Free animals).
Expected Values:628.3ng/ml
Usage:Dissolve with 150ul standard dilutent and let stand for 10 minutes. If powder is partly insoluble, shake gently until fully dissolved. Mix gently before use. In the experiment, add 50ul to an appropriate well of the plate and test its concentration along with standards and your specimens. Note: Upon receipt store Positive Control vial at -20°C. After reconstitution, store Positive Control vial at 4°C and use within 5 days.
Assay Procedure Summary:1. Prepare all reagents, samples and standards
2. Add 50ul standard or sample to each well, then add 50ul prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
3. Aspirate and wash 3 times
4. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C
5. Aspirate and wash 5 times
6. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37°C
7. Add 50ul Stop Solution. Read absorbance at 450nm immediately.
