The Human Anti-Glutamic Acid Decarboxylase Antibodies (Anti-GAD) ELISA Kit is an enzyme immunoassay for the in vitro quantitative measurement of Anti-GAD in human serum, plasma and other biological fluids.
Test Principle:The microtiter plate has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells and the liquid in each well containing unbound material is aspirated after the required incubation period. HRP-conjugated secondary antibody is then added. After incubation the unbound conjugate is washed off. After TMB substrate solution is added, those wells that contain Anti-GAD will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-GAD in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Kit Components:*152277A: Microtiter Plate, 96 wells, Pre-coated, ready to use*152277B: Standard, 2x1vial 152277C: Standard Diluent, 1x20ml*152277D: Detection Reagent A, 1x120ul 152277E: Assay Diluent A, 1x12ml152277F: TMB Substrate, 1x9ml152277G: Stop Solution, 1x6ml152277H: Wash Buffer, 30X, 1x20ml
Storage and Stability:Store *152277A, *152277B and *152277D at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
