Murine VEGF ELISA Cytokine Development kit contains the key components required for the quantitative measurement of natural and/or recombinant murine VEGF in a sandwich ELISA format. Using the ELISA protocol described below, the recommended microplates, reagents and solutions, the components supplied in this kit are sufficient to assay murine VEGF in approximately 1000 ELISA plate wells.
Detection Range:12–1500pg/ml
Crossreactivity:1. When tested at 50ng/ml the following antigens exhibited 100% crossreactivity: Human VEGF121, VEGF165; Rat VEGF
2. When tested at 50ng/ml the following antigens exhibited less than 1% crossreactivity: Human PlGF-2
3. When tested at 50ng/ml the following antigens did not exhibit significant crossreactivity: Human EGF, EGF Receptor, EG-VEGF, flt3L, GM-CSF, PlGF-1, PlGF-3, SCF; Murine EGF, flt3L, GM-CSF, SCF; Rat SCF
145984C: Murine VEGF Standard - 1x1ug of recombinant murine VEGF containing 2.2mg BSA, 11.0mg D-mannitol
145984D: Avidin-HRP Conjugate - 1x60ul
Reconstitution and Storage:145984A: Capture Antibody - Centrifuge vial prior to opening. Reconstitute in 1ml sterile water for a concentration of 100ug/ml
145984B: Detection Antibody - Centrifuge vial prior to opening. Reconstitute in 0.5ml sterile water for a concentration of 100ug/ml 145984C: Murine VEGF Standard - Centrifuge vial prior to opening. Reconstitute in 1ml sterile water for a concentration of 1ug/ml. Note:The reconstituted components are stable for 2 weeks when stored at 2-8°C
Components that have been reconstituted and aliquoted can be stored at -20°C for up to 6 months
145984D: Avidin-HRP Conjugate - Upon receipt, Avidin-HRP conjugate should be aliquoted into ten 6ul vials and stored at -20°C or lower. Aliquots stored frozen at below -20°C are stable for up to 6 months from date of receipt. Avoid more than one freeze-thaw cycle. Avidin should be used in conjunction with ABTS only.
Storage and Stability:Store kit at -20°C. Stable for 6 months after receipt at -20°C. Aliquot and store reconstituted components at -20°C. Upon receipt, aliquot *145984D into ten 6ul vials. Store at -20°C for up to 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.the cap.
仕様
Size：10 Plates
Source Antigen：Mouse
Specificity：When tested at 50ng/ml the following antigens exhibited 100% cross reactivity: Human VEGF 121, VEGF 165 Rat VEGFWhen tested at 50ng/ml the following antigens did not exhibit significantcross reactivity: Human EGF, EG-VEGF, flt3L, GM-CSF, PDGF-AA, PDGF-AB, PDGF-BB, PlGF, SCF Murine EGF, flt3L, GM-CSF, SCF Rat SCF
