High in Normal-1 (HIN-1), also known as Uteroglobin related protein 2 (UGRP2), is an 8kD secreted protein of the secretoglobin superfamily (SCGB3A1) (1). HIN-1 is expressed in bronchial epithelial and secretory Clara cells, mammary epithelial cells (particularly during pregnancy), salivary glands, and the prostate (2-5). It is up-regulated in Clara cells by IL-4, IL-13, Oncostatin M, EGF, and TGF-alpha and downregulated by IFNalpha, beta, and gamma (6-9). It is secreted into bronchial lavage fluid, saliva, and plasma, and may form disulfide-linked dimers (10). HIN-1 binds to the macrophage scavenger receptor MARCO, and to the surface of mammary and bronchial epithelial cells (11, 12). HIN-1 promotes apoptosis and inhibits the proliferation, migration, and invasion of breast cancer cells (12). The down-regulation of HIN-1 expression in many breast, lung, and prostate cancers correlates with hypermethylation of its promoter (4, 10, 13, 14). Mature human HIN-1 shares 58% and 62% aa sequence identity with mouse and rat HIN-1, respectively (4).
Source:Recombinant protein corresponding to Phe21-Gly104 from human HIN-1/SCGB3A1, expressed in E. coli.
Molecular Weight:~6kD
Endotoxin:<0.10EU/1ug (LAL method)
Biological Activity:Measured by the ability of the immobilized protein to support the adhesion of the A549 human lung carcinoma cells. The ED50 for this effect is 0.15-0.75ug/mL.
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute in sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：50ug
Source Antigen：Recombinant, E. coli
Grade：Purified
Purity：~90% (SDS-PAGE)
Form：Supplied as a lyophilized powder in PBS. BSA free. Reconstitute with 200ul sterile PBS.
