Neuronal Pentraxin (NPTX2), also called NP2 or NARP (neuronal activityregulated pentraxin) is a 46-60kD secreted glycoprotein within the Pentraxin family (1-4). Neuronal pentraxins include NPTX1 and NPTX2, both secreted proteins, and NPTXR (Neuronal Pentraxin Receptor), which is found in type II transmembrane or cleaved, soluble forms (1-5). Circulating NPTX2 forms disulfide-linked 250kD homopentamers, while at excitatory synapses it can form heteropentamers and larger clusters with NPTX1 and NPTXR (1-5). The clusters promote synaptogenesis by recruiting subunits of AMPA-type glutamate receptors (AMPAR) (4-6). Of the three neuronal pentraxins, only NPTX2 shows immediate-early expression induced by synaptic activity (5, 6). Human NPTX2 is synthesized as a 431 amino acid (aa) protein with a 15 aa signal sequence and a 416 aa secreted mature protein that contains a calcium binding Pentraxin domain. Mature human NPTX2 shares 96% aa sequence identity with mouse, rat, bovine, and canine NPTX2. Unlike other neuronal pentraxins, NPTX2 expression is not limited to neurons and NPTX2 protein is detected in the plasma (1, 7). It is prominent in the hippocampus, cerebral cortex, cerebellum, hypothalamus, posterior pituitary, and retina (28). It is found at excitatory synapses in parvalbuminexpressing interneurons and vasopressin and orexinexpressing hypothalamic neurons (57). Its expression is increased in the substantia nigra in Parkinson’s disease, and it is present in Lewy bodies (9). NPTX2 shows calciumdependent adhesion (3, 6). It promotes axon outgrowth in cortical explants, and formation, maturation and plasticity of synapses in vivo (36, 8).
Source:Recombinant protein corresponding to aa16-431, Gly16-Leu431, from human Neuronal Pentraxin 2, fused to 6-his tag at C-terminal, NS0-derived.
Molecular Weight:~50-60kD
Endotoxin:<0.01EU/1ug (LAL method)
Biological Activity:Measured by the ability of the immobilized protein to support the adhesion of TT human medullary thyroid cancer cells. The ED50 for this effect is 0.6‑2.4ug/ml.
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute in sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：50ug
Source Antigen：Recombinant, NS0 cells
Grade：Purified
Purity：~90% (SDS-PAGE)
Form：Supplied as a lyophilized powder in PBS. BSA free. Reconstitute with 200ul sterile PBS.
