85-1794-25　MRC1, Recombinant, Rat, aa26-1395 (MMR, CD206, CLEC13D, Macrophage Mannose Receptor, MR) 50ug　145821

特徴

• The MMR (macrophage mannose receptor) is also called MR due to its presence on cells other than macrophages, and is designated CD206, Mrc1 (mannose receptor C type 1), or CLEC13D (C­type lectin domain family 13, member D) (1-4). CD206 is a 175kD endocytic receptor that is expressed on M2 alternatively activated tissue macrophages including tumor­-associated macrophages (TAMs), inflammatory dendritic cells in selected lymphoid organs, and liver, splenic, lymphatic, and dermal microvascular endothelial cells (1, 2, 5­8). The 1463 amino acid (aa) rat CD206 precursor contains a signal sequence (26aa), an extracellular domain (ECD) containing an N­terminal cysteine­rich domain, a fibronectin type II (FNII) domain, eight C­type lectin domains (CTLDs), and several N­glycosylation sites (1369aa), a transmembrane segment and a short (47aa) cytoplasmic domain (2­4).
• Metalloproteinases can mediate the shedding of the soluble ECD (2). The rat CD206 ECD shares 96% aa sequence identity with mouse CD206, and 84­85% with human, equine, porcine and canine CD206. The cysteine­-rich domain recognizes some pituitary hormones such as LH (luteinizing hormone/lutropin) and TSH (thyroid stimulating hormone/thyrotropin), chondroitin sulfates, and sulfated N­acetylgalactosamines including sulfo­Lewisa and ­Lewisx (1, 7, 9).
• The FNII domain mediates Ca2+­independent binding of collagens (2, 10). The CTLDs participate in Ca2+­dependent recognition of branched sugars with terminal mannose, fucose or N­acetylglucosamine that occur on many pathogenic microorganisms (7, 11). CD206 internalizes ligands in clathrin-coated vesicles, sorts them to phagosomes or early endosomes, and recycles to the cell surface (1, 6, 7).
• CD206 also promotes clearance of glycoproteins that promote allergy or ongoing inflammation, such as lysosomal hydrolases and myeloperoxidases (1, 2, 5­7). It is involved in T cell polarization and production of pro- and anti­inflammatory cytokines (1, 2). It facilitates peptide presentation on MHC II, and cross­presentation on MHC I which is important for tumor immunogenicity (1, 2, 12). This function may be blocked by engagement of CD206 on TAMs by tumor mucins (8).
• Source:Recombinant protein corresponding to aa26-1395 from rat MMR/CD206, fused to 6-his tag at C-terminal, NS0-derived, mouse myeloma cell line, Leu26­Ala1395, with a C-­terminal 6­-His tag.
• Molecular Weight:~150-180kD
• Endotoxin:<0.01EU/1ug (LAL method)
• Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.