Matrix metalloproteinase 17 (MMP17) is a zinc metalloproteinase that is an integral membrane protein. It belongs to the membranetype MMP (MTMMP) subfamily, and is also known as MT4MMP. It is associated with the cell membrane by a glycosylphosphatidylinositol (GPI) anchor. It has the potential to function as a protumor necrosis factora (TNFa) convertase. Unlike MMP14 (MT1MMP), it is a poor activator of proMMP2. MMP17 is relatively poor at digesting components of the extracellular matrix, but does cleave fibrinogen and fibrin. MMP17 is known to activate the aggrecanase ADAMTS4 through proteolysis. MMP17 is expressed in many tissues with the highest levels seen in the brain, colon, ovary, testis, and kidney papilla. It has been detected in several human cancers, including gliomas, prostate carcinomas, and breast carcinomas. Recombinant Human MMP17 was expressed without its GPI anchoring sequence, resulting in its secretion as a soluble protein.
Recombinant protein corresponding to Ala39-Ser531 (Arg125Pro) & (Ala182Thr) from human MMP-17, fused to 6X His-Tag at C-terminal, expressed in NS0 cells. N-Terminal Sequence Analysis: Ala39. Swiss/UniProt Accession: Q9ULZ9.
Biological Activity:Measured by its ability to cleave a fluorogenic peptide substrate Mca-PLAQAV-Dpa-RSSSR-NH2
Specific Activity:>150pmol/min/ug, as measured under the described condition
Molecular Weight:~56-65kD, reducing conditions
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：10ug
Source Antigen：Recombinant, NSO cells
Grade：Purified
Purity：≥80% (SDS-PAGE) under reducing conditions and visualized by Colloidal Coomassie Blue Stain at 5ug/lane
Form：Supplied as a liquid in 20mM MES, 800mM sodium chloride, pH 5.5, 4mM CaCl2, 20% glycerol
