The Leptin receptor (Leptin R; gene name LEPR), also called OB R (obesity receptor), is a 150kD protein that is a member of the Class I cytokine receptor family. It mediates the activities of Leptin, a multifunctional hormone produced primarily by adipose tissues that plays roles in food intake, energy metabolism, angiogenesis, reproduction, hematopoiesis, bone metabolism, and immune function (13). The mouse Leptin R gene encodes 1162 amino acids (aa) including a signal peptide, an extracellular region with cytokine receptor homology (CRH), multiple fibronectin type III domains and a WSXWS motif, a transmembrane domain, and a cytoplasmic domain that supports JAK/STAT signaling (2, 3). Mouse Leptin R shares 93% aa sequence identity with rat Leptin R and 74-76% with human, bovine, canine, equine and porcine Leptin R. Leptin R isoforms include a long form, OB RL or OB Rb (primary signaling form), and at least four shorter isoforms with truncated cytoplasmic domains, named OB Ra (ubiquitous), Rc, Rd, and Rf (2, 4). A soluble isoform, OB Re, is found in rodents but not humans (5). However, both rodents and humans produce soluble Leptin R due to release of soluble ectodomains by metalloproteinases such as ADAM10 (5, 6). OB Rb is highly expressed in the hypothalamus and mediates the antiorexigenic effects of Leptin (1, 2). Mutations of ObRb have caused extreme obesity in humans, mice (db/db “diabetes”), and rats (Zucker fa/fa “fatty”) (1, 7-9). Shorter isoforms of Leptin R exhibit limited signaling capability, but mediate endocytosis and degradation of Leptin and passage through the blood-brain barrier (4, 5, 10, 11). Soluble Leptin R is the primary Leptinbinding protein in blood, where it maintains a pool of available bioactive Leptin, delays Leptin clearance from circulation, and downregulates bloodbrain transmission of Leptin (57, 10). In humans, soluble Leptin R levels are inversely proportional to adiposity and are elevated in females versus males (12). Soluble Leptin R is also found upregulated in patients with chronic heart failure, endstage renal disease, and anorexia (13-15). It is expressed by tumorinitiating stem cells, and is proposed as a link between between cancer and obesity (16).
Source:Recombinant protein corresponding to mouse Leptin R Fc Chimera, NS0-derived.
Molecular Weight:~125-190kD
Endotoxin:<0.10EU/1ug (LAL method).
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute in sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：50ug
Source Antigen：Recombinant, NSO cells
Grade：Purified
Purity：~90% (SDS-PAGE)
Form：Supplied as a lyophilized powder in PBS. BSA free. Reconstitute in 500ul sterile PBS.
