Integrin alpha 9 beta 1 is one of twelve integrin family adhesion receptors that share the beta 1 (CD29) subunit (13). It is the noncovalent heterodimer of 150kD alpha 9 and 130kD beta 1 type I transmembrane glycoprotein subunits (3). The alpha 9 extracellular domain (ECD) contains an Nterminal betapropeller structure, followed by domains termed thigh, calf1 and calf2 (2). The beta 1 ECD contains a vWFA domain, which interacts with the alpha 9 betapropeller to form a binding domain when the dimer is in the active, extended and open conformation. The 1006 amino acid (aa) mouse alpha 9 extracellular domain (ECD) shares 95% and 89% aa sequence identity with rat and human alpha 9, respectively, while the 708 aa mouse beta 1 ECD shares 98% aa identity with rat and 9394% with human, bovine, porcine, ovine, canine and feline beta 1. Each subunit has a transmembrane sequence and a short cytoplasmic tail which, for alpha 9, contains binding sites for paxillin and SSAT (spermine/spermidineNacetyltransferase) (4, 5). Integrin alpha 9 beta 1 inhibits cell spreading via signaling through paxillin (4). SSAT interaction with alpha 9 beta 1 allows catalysis that lifts spermine/spermidine inhibition of KIR potassium channels, and is shown to enhance cell migration or suppress airway smooth muscle contraction (5, 6). Lymphangiogenic vascular endothelia growth factors VEGFC and VEGFD are ligands for Integrin alpha 9 beta 1 (7, 8) Genetic deletion of mouse alpha 9 beta 1 results in a fatal chylothorax due to abnormal lymphatic development (9). Integrin alpha 9 beta 1 interacts in cis with GCSF R and enhances its signals, contributing to granulopoiesis (10). It also binds VCAM1, which promotes neutrophil transendothelial migration and delays apoptosis (11, 12). Other ligands for alpha 9 beta 1 include polydom/SVEP1, tenascin, osteopontin, nerve growth factor, and most members of the ADAM family (1316).
Source:Recombinant protein corresponding to mouse Integrin alpha 9 beta 1, CHO-derived.
Molecular Weight:~120-160kD
Endotoxin:<0.10EU/1ug (LAL method).
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute in sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：50ug
Source Antigen：Recombinant
Grade：Highly Purified
Purity：~95% (SDS–PAGE)
Form：Supplied as a lyophilized powder in PBS. Reconstitute in 250ug/ml PBS.
