E6 (Early protein 6) is a viral protein produced in cells infected with the Human Papillomavirus. E6 forms a complex with the host cell ubiquitin ligase E6AP (E6-Associated-Protein) generating a ligase activity that polyubiquitinates tumor suppressors p53 and p73 and targets them to the 26S proteasome for degradation. As a result DNA damage and chromosomal instabilities increase, often leading to cell proliferation and cancer. The E6/E6AP complex also targets other substrates for ubiquitination, such as TERT, BAK1, FADD, and pro-CASP8—none of which appear to be substrates for E6AP in the absence of E6. This protein is untagged, and contains six cysteine-to-serine substitutions at positions 23, 58, 87, 104, 118, and 147 (HPV type 16 sequence numbering).
Recombinant protein corresponding to HPV E6, expressed in E. coli. Contains Cys to Ser substitutions at the following positions: 23, 58, 87, 104, 118 and 147.
Activity:Typical concentration to support E6AP-mediated p53 ubiquitination in vitro is 0.1-2uM depending on experimental conditions.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：25ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：≥98% (SDS-PAGE) under reducing conditions and visualized by Colloidal Coomassie Blue Stain.
Form：Supplied as a liquid in 50mM Hepes, pH 7.0, 400mM sodium chloride, 2mM DTT. BSA free.
