Heparanase (HPSE) selectively cleaves heparan sulfate at specific sites on heparan sulfate proteoglycans (HSPGs) (1, 2, 3, 4). The enzyme is synthesized as an inactive 65kD proenzyme that is secreted via the Golgi apparatus and associates with the cell membrane through interaction with HSPGs (5). It is then endocytosed and transferred to lysosomes (6) where cathepsin L activates it by removing an internal inhibitory peptide, forming a heterodimer composed of an 8kD and a 50kD subunit (7, 8). Under certain stimuli, the active enzyme is transferred back to the cell surface, where it participates in extracellular matrix degradation and remodeling (9). HPSE facilitates cell migration associated with metastasis, wound healing and inflammation (10). An increase in its activity is associated with an increase in VEGF activity, which further enhances angiogenesis (11). HPSE also enhances shedding of syndecans and increases endothelial invasion and angiogenesis in myelomas (12). It acts as a procoagulant by increasing the generation of activation factor X in the presence of tissue factor and activation factor VII (13). In addition, it increases cell adhesion to the extracellular matrix (ECM), independent of its enzymatic activity (14). HPSE is highly expressed in placenta and spleen and weakly expressed in lymph node, thymus, peripheral blood leukocytes, bone marrow, endothelial cells, fetal liver and tumor tissues (15). The enzyme activity of recombinant human HPSE was assayed using 355-labeled recombinant syndecan 4 (16).
Source:Recombinant protein corresponding to Gln36-Ile543 from human active Heparanase/HPSE, fused to His-tag at N-terminal, CHO-derived. The linker peptide from Ser110-Gln157 is removed by protease treatment.
Molecular Weight:~43kD & 9kD
Endotoxin:<1.0EU/1ug (LAL method).
Biological Activity:Measured by its ability to release biotinylated heparan sulfate from Recombinant Human Syndecan‑4. <60 ng of Recombinant Human Active Heparanase/HPSE is required for the release of 50% of heparan sulfate from 2ug of Recombinant Human Syndecan-4, as measured under the described conditions.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
