Frizzled-9 (Fzd9, previously called Fzd3) belongs to the Frizzled family of seven-transmembrane glycoprotein receptors, which are signaling receptors for the family of secreted Wnt ligands (1-3). Mouse Frizzled-9 cDNA encodes aa592 including a 23aa signal peptide, a 207aa extracellular domain (ECD), the 7TM region (aa231-530), and a C-terminal cytoplasmic domain with a PDZ binding motif. The ECD includes a cysteine-rich region (CRD, aa35-156) that binds Wnts and is highly conserved among Frizzleds. Within aa23-186, mouse Frizzled9 shares 100%, 97%, 97% and 96% aa identity with rat, human, canine and bovine Frizzled9, respectively. The human Frizzled-9 gene is within a large region that is deleted in Williams-Beuren syndrome, a developmental disorder affecting personality and cognition (2). Frizzled-9 mRNA is expressed in the mouse central nervous system and myotomes during development, and persists in the hippocampus throughout life (2-4). Adults also show expression in heart, testis, and skeletal muscle (2-4). Cellular expression is detected in proliferating neural precursors, B lymphocyte progenitors, and differentiating osteoblasts (46). Defects in learning, B lymphocyte development, and bone mineralization are observed in mice deleted for Frizzled-9 (4-6). Wnt-2-stimulated signaling through the canonical pathway using LRP5 as a coreceptor is reported, resulting in beta-catenin stabilization and gene transcription (7). However, the canonical pathway is not involved in bone defects of Frizzled-9-/-mice (6). Signaling through the JNK (noncanonical) pathway is shown when Frizzled9-expressing human non-small cell lung cancer cell lines are engaged by Wnt-7a, promoting activation of PPARgamma and expression of cadherin and Sprouty, and reversing transformation (8-10).
Source:Recombinant Fc Chimera protein, a disulfide-linked homodimer, corresponding to Leu24-Asp186 from mouse Frizzled-9 at the N-terminal, Mouse IgG2a, Glu98-Lys330 at C-terminal, expressed in CHO cells.
Molecular Weight:~58-66kD
Endotoxin:<0.01EU/1ug (LAL method)
Biological Activity:Measured by its ability to bind biotinylated recombinant mouse Wnt-3a in a funtional ELISA with an estimated Kd <10 nM.
Storage and Stability:Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：50ug
Source Antigen：Recombinant, CHO cells
Grade：Highly Purified
Purity：≥95% (SDS-PAGE)
Form：Supplied as a lyophilized powder in PBS. BSA free. Reconstitute with 100ul sterile PBS.
