85-1793-90　DCBLD2, Recombinant, Human, aa67-528 (CLCP1, ESDN, Iscoidin, CUB, LCCL Domain Containing Protein 2, Endothelial and Smooth Muscle Cell-derived Neuropilin-li) 50ug　145786

特徴

• DCBLD2 (discoidin, CUB, and LCCL domain containing protein 2), also known as CLCP1 and ESDN, is a 100­130kD glycoprotein that bears structural resemblance to the Neuropilin family of molecules. Mature human DCBLD2 is a type I transmembrane protein that consists of a 462 amino acid (aa) extracellular region (ECD), a 21 aa transmembrane segment, and a 226 aa cytoplasmic domain (1, 2). Within the ECD, human DCBLD2 shares ~88% aa sequence identity with mouse and rat DCBLD2. Alternative splicing generates an isoform that contains a 14 aa insertion in the cytoplasmic domain. DCBLD2 is expressed on vascular smooth muscle cells (VSMC), particularly in regions of arteriosclerosis and vascular injury (2, 3). It is up­regulated on PDGF­BB stimulated VSMC and inhibits PDGF­BB induced VSMC proliferation and migration (2­4). It is also expressed on platelets and inhibits clot formation in a zebrafish model of thrombosis (5). Compared to normal tissue, DCBLD2 expression is down­regulated in some gastric and neuroendocrine tumors but is up­regulated in some metastatic lung cancers (1, 6, 7). It can be tyrosine phosphorylated in response to EGF R signaling and can inhibit colony formation and tumor cell invasiveness (6, 8, 9). Its direct association with Semaphorin 4B induces DCBLD2 degradation (10).
• Source:Recombinant protein corresponding to aa67-528 from human DCBLD2/ESDN, fused to 6-his tag at C-terminal, NS0-derived.
• Molecular Weight:~80-90kD
• Endotoxin:<0.01EU/1ug (LAL method)
• Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute in sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.