The PSG-1, Human BioAssay™ ELISA Kit Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human PSG-1 in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human PSG-1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human PSG-1 showed linear curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human PSG-1.
Intended Use:This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonalantibody specific for PSG-1 has been pre-coated onto a microplate. Standards and samples arepipetted into the wells and any PSG-1 present is bound by the immobilized antibody. Afterwashing away any unbound substances, an enzyme-linked monoclonal antibody specific forPSG-1 is added to the wells. Following a wash to remove any unbound antibody-enzymereagent, a substrate solution is added to the wells and color develops in proportion to theamount of PSG-1 bound in the initial step. The color development is stopped and the intensityof the color is measured.
Specificity:Recognizes natural and recombinant human PSG-1.
Storage and Stability:Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
