Müllerian inhibiting substance (MIS), also named anti-Müllerian hormone (AMH), is a tissue-specific TGF-beta superfamily growth factor. Its expression is restricted to Sertoli cells of fetal and postnatal testis, and to granulosa cells of postnatal ovary. MIS is synthesized and secreted as a disulfide-linked homodimeric propeptide. Proteolytic cleavage is required to separate the N-terminal proregion from the C-terminal bioactive peptide. Both fragments remain associated in a non-covalent complex. The C-terminal sequence forms a cysteine knot via seven canonical cysteine residues found in TGF-beta superfamily members.
Sample Type:Cell culture supernates.
Intended Use:This BioAssay™ DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Mullerian-inhibiting Substance (MIS). DuoSets are designed for the analysis of cell culture supernates.
Range:93.8-6000pg/ml
Specificity:Natural and recombinant human Mullerian-inhibiting Substance (MIS).
Kit Components:Mouse anti-human MIS capture antibody, 1x vialBiotinylated mouse anti-human MIS detection antibody, 1x vialRecombinant human MIS standard, 1x 3vialsStreptavidin-HRP, 1x vial
Storage and Stability:Store other components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
