MFG-E8, originally identified as a product of lactating mammary glands, is also secreted by activated macrophages and immature dendritic cells. MFG-E8 functions as an opsonin by binding phosphatidylserine on apoptotic cells and enhancing their phagocytosis.
Sample Type:Cell culture supernates, serum, EDTA plasma, heparin plasma, saliva, urine and human milk.
Intended Use:The Human MFG-E8 BioAssay™ is a 4.5 hour solid phase ELISA designed to measure MFG-E8 in cell culture supernates, serum, plasma, saliva, urine, and human milk. It contains NS0-expressed recombinant human MFG-E8 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human MFG-E8. Results obtained using natural human MFG-E8 showed linear curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for natural human MFG-E8.
Specificity:Recognizes natural and recombinant human MFG-E8.
Test Principle:This BioAssay™ employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for human MFG-E8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any human MFG-E8 present is bound by the immobilized antibody. After washing away any unbound substances, an enzyme-linked monoclonal antibody specific for human MFG-E8 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of human MFG-E8 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
