Interferon-gamma (IFN-gamma, also known as Type II interferon or immune interferon) is a cytokine produced primarily by T-lymphocytes and natural killer cells. The protein shares no significant homology with IFN-beta or the various IFN-alpha family proteins. Mature IFN-γ exists as noncovalently-linked homodimers. Human IFN-gamma is highly species specific and is biologically active only in human and primate cells. IFN-gamma was originally characterized based on its antiviral activities. The protein also exerts anti-proliferative, immunoregulatory and proinflammatory activities and is thus important in host defense mechanisms. IFN-gamma induces the production of cytokines, up-regulates the expression of class I and II MHC antigens, Fc receptor and leukocyte adhesion molecules. It modulates macrophage effector functions, influences isotype switching and potentiates the secretion of immunoglobulins by B cells. IFN-gamma also augments Helper T cell expansion and may be required for Helper T cell differentiation. IFN-gamma exerts its biological activities by binding to specific cell surface receptors with high-affinity binding sites. The IFN-gamma receptor is present on almost all cell types except mature erythrocytes and has been cloned and characterized. The IFN-gamma receptor is structurally related to the IL-10 receptor.
Intended Use:This BioAssay™ DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant porcine Interferon gamma (IFN-g). DuoSets are designed for the analysis of serum, plasma and cell culture supernates.
Detection Range:62.5-4000pg/ml
Specificity:Recognizes natural and recombinant porcine Interferon gamma (IFN-g).
Storage and Stability:Store 145743A, 145743B and 145743C powder at 4°C. Store reconstituted 145743A and 145743B at -20ºC and are stable for 6 months. Store reconstitued 145743C at -70ºC and is stable for 2 months. Store 145743D at 4ºC and is stable for 6 months.
