Adiponectin (also known as Adipocyte complement-related protein of 30kD, Acrp30) is an adipocyte-derived protein with wide ranging paracrine and endocrine effects on metabolism and inflammation. It promotes adipocyte differentiation, fatty acid catabolism, and insulin sensitivity and is negatively correlated with obesity, type 2 diabetes, and atherogenesis. Adiponectin associates into 70kD noncovalently-linked trimers that are disulfide-linked into medium molecular weight hexamers and then into >300kD high molecular weight oligomers.
Intended Use:The Total Adiponectin/Acrp30, Rat BioAssay™ ELISA Kit is a 2.5 hour solid phase ELISA designed to measure rat Adiponectin levels in cell culture supernates, tissue lysates, serum, plasma, and urine.
Test Principle:The Total Adiponectin/Acrp30, Rat BioAssay™ ELISA Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for rat Adiponectin has been pre-coated onto a microplate. Standards, Control, and samples are pipetted into the wells and any rat Adiponectin present is bound by the immobilized antibody. After washing away any unbound substances, an enzyme-linked polyclonal antibody specific for rat Adiponectin is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells. The enzyme reaction yields a blue product that turns yellow when the Stop Solution is added. The intensity of the color measured is in proportion to the amount of rat Adiponectin bound in the initial step. The sample values are then read off the standard curve.
Storage and Stability:Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
