Interleukin-17A (IL-17A), also known as CTLA-8, is a 15-20kD glycosylated cytokine that plays an important role in anti-microbial and chronic inflammation. The six IL-17 cytokines (IL-17A-F) are encoded by separate genes but adopt a conserved cystine knot fold (1, 2). Mature mouse IL-17A shares 61% and 89% amino acid sequence identity with human and rat IL-17A, respectively (3, 4). IL-17A is secreted by Th17 cells, gamma/delta T cells, iNKT cells, NK cells, LTi cells, neutrophils, and intestinal Paneth cells (2). It forms disulfidelinked homodimers as well as disulfide-linked heterodimers with IL-17F (5, 6). IL-17A exerts its effects through the transmembrane IL-17 RA in complex with IL-17 RC or IL-17 RD (7, 8). Both IL-17 RA and IL-17 RC are required for responsiveness to heterodimeric IL-17A/F (7). IL-17A promotes protective mucosal and epidermal inflammation in response to microbial infection (9-12). It induces chemokine production, neutrophil influx, and the production of antibacterial peptides (9-11). IL-17A/F likewise induces neutrophil migration, but IL-17F does not (11). IL-17A additionally enhances the production of inflammatory mediators by rheumatoid synovial fibroblasts and contributes to TNF-alpha induced shock (Fossiez, 14). In contrast, it can protect against the progression of colitis by limiting chronic inflammation (12). IL-17A encourages the formation of autoreactive germinal centers and exacerbates the onset and progression of experimental models of autoimmunity (15, 16). IL-17A has been shown to exert either tumorigenic or anti-tumor effects (17, 18).
Source:Recombinant protein corresponding to aa26-158 from mouse IL-17A, CHO-derived.
Molecular Weight:~15-22kD
Endotoxin:<0.01EU/1ug (LAL method)
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：25ug
Source Antigen：Recombinant
Grade：Highly Purified
Purity：~95% (SDS-PAGE)
Form：Supplied as a lyophilized powder in PBS, BSA. Reconstitute in 100ug/ml in PBS (>0.1% human or BSA).
