Interleukin10 Receptor beta (IL10 R beta), also known as IL10 R2 and CRF24, is a 60kD transmembrane glycoprotein that functions as a coreceptor for several class 2 cytokines including Interleukins10, 22, 26, 28A/IFNgamma2, 28B/IFNgamma3, and 29/IFNgamma (1, 2). IL10 R beta associates with ligandspecific receptor subunits to form signaling receptor complexes, e.g. IL10 R alpha for IL10 (3, 4), IL20 R alpha for IL26 (5, 6), IL22 R alpha for IL22 (7, 8), and IL28 R alpha for IL28A, IL28B, and IL29 (9, 10). IL10 R beta is widely expressed, while the associated receptor subunits exhibit differential expression patterns (1). The ligandspecific subunits are responsible for the divergent functions of these cytokines, encompassing immune suppression, promotion or inhibition of inflammation, mucosal defense, antiviral immunity, and hematopoiesis (1). IL10 R beta deficient mice lack responsiveness to each of those cytokines. IL10 R beta contributes to ligand binding, but effective signaling is only triggered in the presence of the ligandspecific subunit (8, 9, 11). In the case of IL10, a cytokine dimer binds to two IL10 R alpha/IL10R1 chains, resulting in recruitment of two IL10 R beta/IL10R2 chains (3, 12). Some members of the IL10 family are monomeric cytokines and interact with single molecules of IL10 R beta and their ligandspecific subunit (1). Mature mouse IL10 R beta consists of a 201 amino acid (aa) extracellular region with two fibronectin typeIII domains, a 21 aa transmembrane segment and a 108 aa cytoplasmic domain (13). Within the ECD, mouse IL10 R beta shares 75% and 87% aa sequence identity with human and rat IL10 R beta, respectively.
Source:Recombinant protein corresponding to aa21-220 from IL-10 R beta Fc Chimera.
Molecular Weight:~7080kD
Endotoxin:<0.10EU/1ug (LAL method).
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute in sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：50ug
Source Antigen：Recombinant
Grade：Highly Purified
Purity：~95% (SDS-PAGE)
Form：Supplied as a lyophilized powder in PBS, Trehalose. BSA free. Reconstitute in 100ug/ml PBS.
