Vascular endothelial growth factor receptor 2 (VEGFR-2) also known as Kinase insert domain receptor (KDR, a type III receptor tyrosine kinase) is a VEGF receptor. Through in situ hybridization of a genomic DNA probe to metaphase chromosomes, VEGFR2 was localized to 4q11-->q12. VEGF receptor 2 and the adherens junction act as shear-stress cotransducers, mediating the transduction of shear-stress signals into vascular endothelial cells.
Intended Use:VEGFR2/KDR BioAssay™ ELISA Kit is a sandwich ELISA for quantitative detection of human VEGFR2 in cell culture supernates, serum and plasma (heparin, EDTA).
Range: 62.5-4000pg/ml
Sensitivity: <4pg/ml
Specificity: Natural and recombinant human VEGFR2
Crossreactivity: No detectable crossreactivity with other relevant proteins
Storage and Stability:Store *144099B powder at 4°C. Once reconstituted store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Assay Summary:1. Add samples and standards and incubate the plate at 37°C for 90 minutes. Do not wash. 2. Add biotinylated antibodies and incubate the plate at 37°C for 60 minutes. Wash plate 3 times with 0.01M TBS
3. Add ABC working solution and incubate the plate at 37°C for 30 minutes. Wash plate 5 times with 0.01M TBS
4. Add TMB color developing agent and incubate the plate at 37°C in dark for 25-30 minutes
