85-1792-47　TNFSF11/RANKL BioAssay™ ELISA Kit, Mouse 96Tests　144077

特徴

• Sandwich ELISA quantitative detection kit for Mouse TNFSF11/RANKL in cell culture supernates, serum and plasma (heparin, EDTA).
• Receptor activator of nuclear factor kappa-B ligand(RANKL), also known as tumor necrosis factor ligand superfamily member 11 (TNFSF11), is a protein that in humans is encoded by the TNFSF11 gene. This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This gene is mapped to chromosome13q14.11. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. This gene may play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.
• Assay Range:62.5pg/ml-4000pg/ml
• Sensitivity: < 10 pg/ml
• Crossreactivity:There is no detectable cross-reactivity with other relevant proteins.
• Uniprot ID:O35235
• Entrez Gene ID:21943
• Kit Components:96-well plate pre-coated with anti-TNFSF11/RANKL, MouseLyophilized recombinant TNFSF11/RANKL, Mouse standard, 10ng/tube, 2EaUSB Cat No Kit Component143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)143697 Sample diluent buffer, 30ml143698 Antibody diluent buffer, 12ml143699 ABC diluent buffer, 12ml143700 TMB color developing agent, 10ml143701 TMB stop solution, 10ml
• Storage and Handling:Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles.