85-1792-36　TNF alpha BioAssay™ ELISA Kit, Mouse 96Tests　144066

特徴

• Sandwich ELISA quantitative detection kit for Mouse TNF alpha in cell culture supernates, serum and plasma (heparin, EDTA).
• Tumor necrosis factor-alpha (TNF-alpha, or TNF) is secreted by macrophages in response to inflammation, infection and cancer.1 Human Tumor Necrosis Factor (TNF) and Lymphotoxin (TNF-beta) are cytotoxic proteins which have similar biological activities and share 30% amino acid homology.2 TNF-alpha is produced by monocytes, which can stimulate endothelial cells to produce the multilineage growth factor granulocyte-macrophage colony-stimulating factor and extend the role of this immunoregulatory protein to the regulation of hematopoiesis in vitro.3 TNF is a soluble protein that causes damage to tumor cells but has no effect on normal cells. Human TNF has been purified to apparent homogeneity as a 17.3-kilodalton protein from HL-60 leukemia cells and has showed cytotoxic and cytostatic activities against various human tumor cell lines. The human TNF cDNA is 1585 base pairs in length and encodes a protein of 233 amino acids. The mature protein begins at residue 77, leaving a long leader sequence of 76 amino acids.4 TNF-alpha has been mapped to human chromosome 6.2
• Assay Range:15.6pg/ml-1000pg/ml (Body fluids, tissue lysates or cell culture supernates), 7.8pg/ml-500pg/ml (Mouse serum)(Body fluids, tissue lysates or cell culture supernates)7.8pg/ml-500pg/ml(Mouse serum)
• Sensitivity:< 1 pg/ml
• Crossreactivity:There is no detectable cross-reactivity with other relevant proteins.
• Uniprot ID:P06804
• Entrez Gene ID:21926
• Kit Components:96-well plate pre-coated with anti-TNF alpha, MouseLyophilized recombinant TNF alpha, Mouse standard, 10ng/tube, 2EaUSB Cat No Kit Component143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)143697 Sample diluent buffer, 30ml143698 Antibody diluent buffer, 12ml143699 ABC diluent buffer, 12ml143700 TMB color developing agent, 10ml143701 TMB stop solution, 10ml
• Storage and Handling:Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles.