sVEGFR1 is also known as sFMS-related tyrosine kinase 1(sFLT1). Oncogene sFLT belongs to the src gene family and is related to oncogene ROS . Like other members of this family, it shows tyrosine protein kinase activity that is important for the control of cell proliferation and differentiation. sFLT is maps to 13q12. sVEGF receptor 1 signaling is essential for osteoclast development and bone marrow formation in colony-stimulating factor 1-deficient mice. The standard product used in this kit is recombinant human sVEGFR1, consisting of 905 amino acids with a molecular mass of 100kD.
Intended Use:sVEGFR1/sFLT1 BioAssay™ ELISA Kit, Human is a sandwich ELISA quantitative detection of human sVEGFR1/sFLT1 in cell culture supernates, serum and plasma (EDTA).
Range:15.6-10,000pg/ml
Sensitivity:<30pg/ml
Specificity:Natural and recombinant human sVEGFR1/sFLT1
Crossreactivity:No detectable crossreactivity with other relevant proteins
Storage and Stability:Store *144047B powder at 4°C. Once reconstituted store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Assay Summary:1. Add samples and standards and incubate the plate at 37°C for 90 minutes. Do not wash. 2. Add biotinylated antibodies and incubate the plate at 37°C for 60 minutes. Wash plate 3 times with 0.01M TBS
3. Add ABC working solution and incubate the plate at 37°C for 30 minutes. Wash plate 5times with 0.01M TBS
4. Add TMB color developing agent and incubate the plate at 37°C in dark for 25-30 minutes
