85-1792-09　sFAS BioAssay™ ELISA Kit, Mouse 96Tests　144039

特徴

• Sandwich ELISA quantitative detection kit for Mouse Soluble FAS in cell culture supernates, serum and plasma (heparin, EDTA, citrate).
• Fas, also known as APO-1, CD95 and TNFRSF6, is a member of the nerve growth factor (NGF)/tumor necrosis factor (TNF) receptor superfamily and mediates apoptosis.1 The nucleotide sequence of the cDNAs reveales that the molecule coding for the Fas antigen determinant is a 319 amino acid polypeptide with a single transmembrane domain. The extracellular domain is rich in cysteine residue, and shows a similarity to that of human tumor necrosis factor receptors, human nerve growth factor receptor, and human B cell antigen CD40.2 The APO-1 antigen as defined by the mouse monoclonal antibody anti-APO-1 is previously found to be expressed on the cell surface of activated human T and B lymphocytes and a variety of malignant human lymphoid cell lines. The APO-1 antigen is found to be a membrane glycoprotein of 48kD.3 Fas antigen is expressed and functional on papillary thyroid cancer cells and this may have potential therapeutic significance.4 Fas can play a role as an inducer of both neurite growth in vitro and accelerates recovery after nerve injury in vivo.5
• Assay Range:31.2pg/ml-2000pg/ml
• Sensitivity:< 3 pg/ml
• Crossreactivity:There is no detectable cross-reactivity with other relevant proteins.
• Uniprot ID:Q8C350
• Entrez Gene ID:14102
• Kit Components:96-well plate pre-coated with anti-sFAS, MouseLyophilized recombinant sFAS, Mouse standard, 10ng/tube, 2EaUSB Cat No Kit Component143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)143697 Sample diluent buffer, 30ml143698 Antibody diluent buffer, 12ml143699 ABC diluent buffer, 12ml143700 TMB color developing agent, 10ml143701 TMB stop solution, 10ml
• Storage and Handling:Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles.