85-1791-54　MMP-9 BioAssay™ ELISA Kit, Human 96Tests　143983

特徴

• Sandwich ELISA quantitative detection kit for Human MMP-9 in cell culture supernates, serum, plasma (heparin), saliva and urine.
• The 92kD type IV collagenase is also known as 92kD gelatinase, type V collagenase, gelatinase B, or matrix metalloproteinase-9 (MMP9). The 72- and 92kD type IV collagenases are members of a group of secreted zinc metalloproteases.1 The matrix metalloproteinases (MMPs) are able to degrade the extracellular matrix and allow angiogenesis and tumor invasion.2 Gelatinase B, a matrix metalloproteinase that has proteolytic activity against connective tissue proteins, has been suggested to be important in the connective tissue remodeling processes associated with atherogenesis and plaque rupture.3 MMP-9 is predominantly expressed in neutrophils, macrophages, and mast cells, rather than in oncogene-positive neoplastic cells.4 The polymorphism of MMP-9 acts as a genetic factor for the development of smoking-induced pulmonary emphysema.5 The standard product used in this kit is recombinant human MMP-9 with the molecular mass of 95kD. The detected MMP-9 includes zymogen and active enzyme.
• Assay Range:156pg/ml-10,000pg/ml
• Sensitivity:< 5 pg/ml
• Crossreactivity:There is no detectable cross-reactivity with other relevant proteins.
• Uniprot ID:P14780
• Entrez Gene ID:4318
• Kit Components:96-well plate pre-coated with anti-MMP-9, HumanLyophilized recombinant MMP-9, Human standard, 10ng/tube, 2EaUSB Cat No Kit Component143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)143697 Sample diluent buffer, 30ml143698 Antibody diluent buffer, 12ml143699 ABC diluent buffer, 12ml143700 TMB color developing agent, 10ml143701 TMB stop solution, 10ml
• Storage and Handling:Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles.