85-1791-41　MIP-1 alpha BioAssay™ ELISA Kit, Human 96Tests　143970

特徴

• Sandwich ELISA quantitative detection kit for Human MIP-1 alpha in cell culture supernates, serum and plasma (heparin, EDTA, citrate).
• Macrophage inflammatory protein-1 alpha (MIP-1 alpha), also called CCL3, LD78. The cDNA for MIP-1 alpha predicts a mature peptide of 69 amino acids with a molecular mass of 7,889 daltons.1 LD78 is a member of a newly identified superfamily of small inducible proteins involved in inflammatory responses, wound healing and tumorigenesis. MIP-1 alpha is a chemokine that has pro-inflammatory and stem cell inhibitory activities in vitro. It constitutes an important second signal for mast cell degranulation in the conjunctiva in vivo and consequently for acute-phase disease. The standard product used in this kit is recombinant human MIP-1α, consisting of 66 amino acids with the molecular mass of 7.5kD.
• Assay Range:31.2pg/ml-2000pg/ml
• Sensitivity:< 10 pg/ml
• Crossreactivity:There is no detectable cross-reactivity with other relevant proteins.
• Uniprot ID:A0N0R1
• Entrez Gene ID:6348
• Kit Components:96-well plate pre-coated with anti-MIP-1 alpha, HumanLyophilized recombinant MIP-1 alpha, Human standard, 10ng/tube, 2EaUSB Cat No Kit Component143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)143697 Sample diluent buffer, 30ml143698 Antibody diluent buffer, 12ml143699 ABC diluent buffer, 12ml143700 TMB color developing agent, 10ml143701 TMB stop solution, 10ml
• Storage and Handling:Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles.