Interleukin-6 (IL-6) is a protein that in humans is encoded by the IL6 gene.1 Interleukin-6 (IL-6) is involved not only in the hepatic acute phase response but also in adipose tissue metabolism, lipoprotein lipase activity, and hepatic triglyceride secretion.2 IL-6 is a pleiotropic cytokine that plays a critical role in bone resorption.3 IL-6 has been suggested to stimulate the HPA axis during immune activation independent of the input of hypothalamic corticotropin-releasing hormone (CRH).4 Interleukin-6 (IL-6) is an immunoregulatory cytokine that activates a cell-surface signaling assembly composed of IL-6, the IL-6 alpha-receptor (IL-6Ralpha), and the shared signaling receptor gp130.5 The standard product used in this kit is recombinant rat IL-6, consisting of 188 amino acids with the molecular mass of 21.8kD.
Intended Use:IL-6 BioAssay™ ELISA Kit is a sandwich ELISA for quantitative detection of rat IL-6 in cell culture supernates, serum and plasma (heparin, EDTA, citrate).
Range: 62.5-4000pg/ml
Sensitivity: <5pg/ml
Specificity: Natural and recombinant rat IL-6
Crossreactivity:No detectable crossreactivity with other relevant proteins
Storage and Stability:Store *143931B powder at 4°C. Once reconstituted store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Assay Summary:1. Add samples and standards and incubate the plate at 37°C for 90 minutes. Do not wash. 2. Add biotinylated antibodies and incubate the plate at 37°C for 60 minutes. Wash plate 3 times with 0.01M TBS or PBS
3. Add ABC working solution and incubate the plate at 37°C for 30 minutes. Wash plate 5 times with 0.01M TBS or PBS
4. Add TMB color developing agent and incubate the plate at 37°C in dark for 20-25 minutes
