85-1791-03　Interleukin 5, Mouse, BioAssay™ ELISA Kit (IL-5, Interleukin-5, B Cell Differentiation Factor I, T-cell Replacing Factor, TRF) 96Tests　143928

特徴

• Sandwich ELISA quantitative detection kit for Mouse IL-5 in cell culture supernates and serum.
• Interleukin-5 (IL-5) is also known as eosinophil differentiation factor (EDF). IL-5 is a potential candidate gene in the pathogenesis of asthma, as it is the main cytokine controlling eosinophil activity and eosinophils are pivotal in the development of airway inflammation.1 The predicted amino acid sequence of 134 amino acids is identical with that recently reported for human interleukin-5 but shows no significant homology with other known hemopoietic growth regulators.2 IL-5 is a lineage-specific hematopoietic growth factor that stimulates the production of eosinophils and eosinophil colonies from normal human bone marrow cells. IL-5 gene to human chromosome 5 at bands q23-31.3 Human and mouse of IL-5 share 70% amino acid sequence homology.
• Assay Range:15.6pg/ml-1000pg/ml
• Sensitivity:< 2 pg/ml
• Crossreactivity:There is no detectable cross-reactivity with other relevant proteins.
• Uniprot ID:P04401
• Entrez Gene ID:16191
• Kit Components:96-well plate pre-coated with anti-IL-5, MouseLyophilized recombinant IL-5, Mouse standard, 10ng/tube, 2EaUSB Cat No Kit Component143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)143697 Sample diluent buffer, 30ml143698 Antibody diluent buffer, 12ml143699 ABC diluent buffer, 12ml143700 TMB color developing agent, 10ml143701 TMB stop solution, 10ml
• Storage and Handling:Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles.