Interleukin-27 (IL-27) is a heterodimeric cytokine belonging to the IL-12 family that is composed of two subunits; Epstein-Barr virus (EBV)-induced gene 3 (EBI3) (also known as IL-27B) and IL27-p28 (known as IL-30). IL-27 is produced by antigen-presenting cells.IL-27 plays an important function in regulating the activity of B- and T-lymphocytes. IL-27, a potent inhibitor of T(H)-17 cell development, may be a useful target for treating inflammatory diseases mediated by these cells.
Intended Use:For quantitative detection of human IL-27 in cell culture supernates, serum and plasma (heparin, EDTA, citrate).
Range:156pg/ml-10,000pg/ml
Sensitivity:<10pg/ml
Specificity:No detectable crossreactivity with other cytokine.
Storage and Stability:Store *143747B powder at 4°C. Once reconstituted store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Assay Summary:1. Add 100ul of samples and standards and incubate the plate at 37°C for 90 min. Do not wash
2. Add 100ul biotinylated antibody and incubate the plate at 37°C for 60 min. Wash plate 3 times with 0.01M TBS or PBS
3. Add ABC working solution and incubate the plate at 37°C for 30min. Wash plate 5 times with 0.01M TBS or PBS
4. Add TMB color developing agent and incubate the plate at 37°C in dark for 20-25 min
