Interleukin-2 (IL2), formerly referred to as T-cell growth factor, is a powerfully immunoregulatory lymphokine that is produced by lectin- or antigen-activated T cells. It is produced not only by mature T lymphocytes on stimulation but also constitutively by certain T-cell lymphoma cell lines. The lymphokine interleukin-2 (IL-2) is responsible for autocrine cell cycle progression and regulation of immune responses. IL-2 expression in mature thymocytes and T cells has been found to be tightly controlled by monoallelic expression.1 IL-2 can act as a growth hormone for both B and T lymphocytes.2 The human gene for interleukin 2 (IL2) is assigned to chromosome 4.3 Human IL-2 is a 15.5kD glycoprotein, consisting of 153 amino acids in precursor form and 133 amino acids in mature form.
The IL-2 BioAssay™ ELISA Kit is a sandwich ELISA for quantitative detection of human IL-2 in cell culture supernates, serum and plasma (heparin, EDTA, citrate).
Test Principle:The Human IL-2 BioAssay™ ELISA Kit, is a solid phase immunoassay specially designed to measure human IL-2 with a 96-well strip plate that is pre-coated with antibody specific for IL-2. The detection antibody is a biotinylatedantibody specific for IL-2. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant human IL-2 with immunogen: Expression system for standard: E. coli, A21-T153.
To measure human IL-2, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex. Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to human IL-2 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of human IL-2 in the sample.
Assay Range:15.6-1000pg/ml
Sensitivity:<1 pg/ml
Specificity:Natural and recombinant human IL-2
Crossreactivity:There is no detectable cross-reactivity with other relevant proteins.
Storage and Stability:Store all components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
