85-1790-71　IL-13 BioAssay™ ELISA Kit, Mouse 96Tests　143896

特徴

• Sandwich ELISA quantitative detection kit for Mouse IL-13 in cell culture supernates, serum and plasma (heparin, EDTA).
• Interleukin (IL)-13 is a major inducer of fibrosis in many chronic infectious and autoimmune diseases.1 Recombinant IL-13 protein inhibits inflammatory cytokine production induced by lipopolysaccharide in human peripheral blood monocytes. Moreover, it synergizes with IL-13 in regulating interferon-gamma synthesis in large granular lymphocytes. Interleukin-13 may be critical in regulating inflammatory and immune responses.2 IL-13 and IL-4 are two cytokines produced by T helper type 2 cells, mast cells, and basophils. In addition to their physiological roles, these cytokines are also implicated in pathological conditions such as asthma and allergy.3 The genes encoding human IL-4 and IL-13 are located on segment q23-31 of chromosome 5.4 Recombinant mouse IL-13 is a mixture of Ser26-Phe131 amino acids with the molecular mass of 11.5kD.
• Assay Range:15.6pg/ml-1000pg/ml
• Sensitivity:< 2 pg/ml
• Crossreactivity:There is no detectable cross-reactivity with other relevant proteins.
• Uniprot ID:P20109
• Entrez Gene ID:16163
• Kit Components:96-well plate pre-coated with anti-IL-13, MouseLyophilized recombinant IL-13, Mouse standard, 10ng/tube, 2EaUSB Cat No Kit Component143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)143697 Sample diluent buffer, 30ml143698 Antibody diluent buffer, 12ml143699 ABC diluent buffer, 12ml143700 TMB color developing agent, 10ml143701 TMB stop solution, 10ml
• Storage and Handling:Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles.