85-1790-70　IL-13 BioAssay™ ELISA Kit, Rat 96Tests　143895

特徴

• Sandwich ELISA quantitative detection kit for Rat IL-13 in cell culture supernates, serum and plasma (heparin, EDTA).
• Interleukin 13 (IL-13) is a protein that in humans is encoded by the IL13 gene. IL-13 is cytokine secreted by many cell types, but especially T helper type 2 (Th2) cells, that is an important mediator of allergic inflammation and disease. IL-13 induces its effects through a multi-subunit receptor that includes the alpha chain of the IL-4 receptor (IL-4Rα) and at least one of two known IL-13-specific binding chains. Most of the biological effects of IL-13, like those of IL-4, are linked to a single transcription factor, signal transducer and activator of transcription 6 (STAT6). The IL13 gene is clustered with IL3, IL5, IL4, and CSF2 on 5q. Physical maps of the 5q23-q31 region show the following order: cen--IL3--CSF2--IL13--IL4--IL5--tel, with IL13 particularly close to IL4.
• Assay Range:15.6pg/ml-1000pg/ml
• Sensitivity:< 1 pg/ml
• Crossreactivity:There is no detectable cross-reactivity with other relevant proteins.
• Uniprot ID:P42203
• Entrez Gene ID:116553
• Kit Components:96-well plate pre-coated with anti-IL-13, RatLyophilized recombinant IL-13, Rat standard, 10ng/tube, 2EaUSB Cat No Kit Component143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)143697 Sample diluent buffer, 30ml143698 Antibody diluent buffer, 12ml143699 ABC diluent buffer, 12ml143700 TMB color developing agent, 10ml143701 TMB stop solution, 10ml
• Storage and Handling:Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles.