85-1790-60　Interleukin-1 beta, Human, BioAssay™ ELISA Kit (IL-1 beta, Catabolin, IL-1B, IL1F2, IL1-beta) 96Tests　143885

特徴

• Sandwich ELISA quantitative detection kit for Human IL-1 beta in cell culture supernates, serum and plasma (heparin, EDTA, citrate).
• Interleukin-1b ( IL-1b) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1b, a prominent microglia-derived cytokine, caused oligodendrocyte death in coculture with astrocytes and microglia, but not in pure culture of oligodendrocytes alone. It also can cause nuclear export of a specific NCOR corepressor complex, resulting in derepression of a specific subset of nuclear factor-kappa-B (NFKB)-regulated genes2. Furthermore, Microenvironmental IL-1b and, to a lesser extent, IL-1a are required for in vivo angiogenesis and invasiveness of different tumor cells. Additional, the cooperation of IL-1b and PDGFB induces contractile-to-synthetic phenotype modulation of human aortic smooth muscle cells in culture. Moreover, the association with disease may be explained by the biologic properties of IL-1b, which is an important proinflammatory cytokine and a powerful inhibitor of gastric acid secretion.
• Assay Range:3.9-250pg/ml
• Sensitivity:<0.15 pg/ml
• Specificity:Recognizes native and recombinant human IL-1b.
• Crossreactivity:There is no detectable cross-reactivity with other relevant proteins.
• Uniprot ID:P01584
• Entrez Gene ID:3553
• Kit Components:143885A: Microtiter Strip, 1x96-well Pre-coated with anti-IL-1 beta, Human 143885B: Standard, 1000pg/ml, 2x1vial Lyophilized recombinant IL-1 beta, Human 143885C: Pab (Biotin) GtxHu, 1x130ulUSB Cat NoKit Component143696: Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (1:100 dilution)143697: Sample diluent buffer, 1x30ml143698: Antibody diluent buffer, 1x12ml143699: ABC diluent buffer, 1x12ml143700: TMB color developing agent, 1x10ml143701: TMB stop solution, 2N H2SO4, 1x10ml
• Storage and Stability:Store *143885B powder at 4°C. Once reconstituted store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Summary1. Add samples and standards and incubate the plate at 37°C for 90 minutes. Do not wash. 2. Add biotinylated antibodies and incubate the plate at 37°C for 60 minutes. Wash plate 3 times with 0.01M TBS. 3. Add ABC working solution and incubate the plate at 37°C for 30 minutes. Wash plate 5 times with 0.01M TBS. 4. Add TMB color developing agent and incubate thutese plate at 37°C in dark for 25-30 min. 5. Add TMB stop solution and read.