85-1790-43　ICAM-1 BioAssay™ ELISA Kit, Mouse 96Tests　143865

特徴

• Sandwich ELISA quantitative detection kit for Mouse ICAM-1 in cell culture supernates, serum, plasma (heparin, EDTA) and tissue homogenates.
• Intercellular adhesion molecule-1 (ICAM-1) is an integral membrane protein, a member of the immunoglobulin superfamily, and a ligand for lymphocyte function-associated (LFA) antigens, a beta 2 leukocyte integrin.1 The normal function of human intercellular adhesion molecule-1 (ICAM-1) is to provide adhesion between endothelial cells and leukocytes after injury or stress. ICAM-1 binds to leukocyte function-associated antigen (LFA-1) or macrophage-1 antigen (Mac-1).2 ICAM-1 is found on leukocytes, fibroblasts, epithelial cells and endothelial cells and its expression is regulated by inflammatory cytokines.3 ICAM-1 has a tissue distribution similar to that of the major histocompatibility complex class II antigens and is likely to play a role in inflammatory responses.1 The standard product used in this kit is produced by recombinant gene expression, which is a dimeric protein linked with disulfide bonds. The single chain is Gln28-Asn 485. It has 457 amino acids with the molecular mass of 76.8kD. As a result of glycosylation, the molecular mass of 110-130kD is revealed by SDS-PAGE.
• Assay Range:156pg/ml-10,000pg/ml
• Sensitivity:< 10 pg/ml
• Crossreactivity:There is no detectable cross-reactivity with other relevant proteins.
• Uniprot ID:P13597
• Entrez Gene ID:15894
• Kit Components:96-well plate pre-coated with anti-ICAM-1, MouseLyophilized recombinant ICAM-1, Mouse standard, 10ng/tube, 2EaUSB Cat No Kit Component143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)143697 Sample diluent buffer, 30ml143698 Antibody diluent buffer, 12ml143699 ABC diluent buffer, 12ml143700 TMB color developing agent, 10ml143701 TMB stop solution, 10ml
• Storage and Handling:Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles.