Sandwich ELISA quantitative detection kit for Human Elafin/PI3 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
Elafin, also known as peptidase inhibitor 3 or skin-derived antileukoproteinase (SKALP) is a protein that in humans is encoded by the PI3 gene. It is mapped to 20q13.12. This gene encodes an elastase-specific inhibitor that functions as an antimicrobial peptide against Gram-positive and Gram-negative bacteria. The protein contains a WAP-type four-disulfide core (WFDC) domain, and is thus a member of the WFDC domain family. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. This gene belongs to the centromeric cluster. Expression of this gene is upgulated by bacterial lipopolysaccharides and cytokines. This gene is the neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis.
Assay Range:31.2-2000pg/ml
Sensitivity:<10pg/ml
Uniprot ID:P19957
Entrez Gene ID:5266
Specificity:Natural and recombinant human Elafin/PI3.
Crossreactivity:There is no detectable cross-reactivity with other relevant proteins.
Storage and Stability:Store *143816B powder at 4°C. Once reconstituted store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
