85-1789-88　EG-VEGF BioAssay™ ELISA Kit, Human 96Tests　143811

特徴

• Sandwich ELISA quantitative detection kit for Human EG-VEGF in cell culture supernates, serum and plasma (heparin, EDTA).
• EG-VEGF，Endocrine glandderived vascular endothelial growth factor, is a human protein encoded by by the PROKR1 gene. Its gene is mapped to chromosome 1. The 1.4-kb cDNA encodes a protein of 105 amino acids with a well defined signal sequence. The mature protein is predicted to have 86 amino acids. Northern blot analysis demonstrated expression in testis, ovary, adrenal gland, and placenta. EG-VEGF has played a vital role in inducing proliferation, migration, and fenestration in capillary endothelial cells derived from endocrine glands. The standards of this kit are recombinant human EG-VEGF (A20-F105), with molecular weight of 10.5kD.
• Assay Range:15.6pg/ml-1000pg/ml
• Sensitivity:< 7 pg/ml
• Crossreactivity:There is no detectable cross-reactivity with other relevant proteins.
• Uniprot ID:P58294
• Entrez Gene ID:84432
• Kit Components:96-well plate pre-coated with anti-EG-VEGF, HumanLyophilized recombinant EG-VEGF, Human standard, 10ng/tube, 2EaUSB Cat No Kit Component143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)143697 Sample diluent buffer, 30ml143698 Antibody diluent buffer, 12ml143699 ABC diluent buffer, 12ml143700 TMB color developing agent, 10ml143701 TMB stop solution, 10ml
• Storage and Handling:Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles.