85-1789-79　Cystatin C BioAssay™ ELISA Kit, Human 96Tests　143802

特徴

• Sandwich ELISA quantitative detection kit for Human Cystatin C in cell culture supernates, serum, plasma ( eparin, EDTA), saliva, urine and human milk.
• Cystatin C or cystatin 3 (formerly gamma trace, post-gamma-globulin or neuroendocrine basic polypeptide), a protein encoded by the CST3 gene, was originally described as a constituent of normal cerebrospinal fluid (CSF) and of urine from patients with renal failure.1 Cystatin 3 has a low molecular weight (approximately 13.3 kilodaltons), and it is removed from the bloodstream by glomerular filtration in the kidneys. In humans, all cells with a nucleus (cell core containing the DNA) produce cystatin C as a chain of 120 amino acids. It is found in virtually all tissues and bodily fluids. Cystatin C, which belongs to the type II cystatin gene family, is a potent inhibitor of lysosomal proteinases2 (enzymes from a special subunit of the cell that break down proteins) and probably one of the most important extracellular inhibitors of cysteine proteases3 (it prevents the breakdown of proteins outside the cell by a specific type of protein degrading enzymes). Moreover, cystatin C is involved in network reorganization in the epileptic dentate gyrus.4
• Assay Range:312pg/ml-20,000pg/ml
• Sensitivity:< 10 pg/ml
• Crossreactivity:There is no detectable cross-reactivity with other relevant proteins.
• Uniprot ID:P01034
• Entrez Gene ID:1471
• Kit Components:96-well plate pre-coated with anti-Cystatin C, HumanLyophilized recombinant Cystatin C, Human standard, 10ng/tube, 2EaUSB Cat No Kit Component143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)143697 Sample diluent buffer, 30ml143698 Antibody diluent buffer, 12ml143699 ABC diluent buffer, 12ml143700 TMB color developing agent, 10ml143701 TMB stop solution, 10ml
• Storage and Handling:Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles.