85-1789-41　CD25/IL-2sR alpha BioAssay™ ELISA Kit, Human 96Tests　143764

特徴

• Sandwich ELISA quantitative detection kit for Human CD25/IL-2sR alpha in cell culture supernates, serum and plasma (heparin, EDTA).
• CD25 is the alpha chain of the IL-2 receptor. It is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes that associates with CD122 to form a heterodimer that can act as a high-affinity receptor for IL-2. The IL2R gene was mapped to 10p15-p14. CD25 is expressed in most B-cell neoplasms, some acute nonlymphocytic leukemias, neuroblastomas, and tumor infiltrating lymphocytes. Its soluble form, called sIL-2R may be elevated in these diseases and is occasionally used to track disease progression.
• Assay Range: 78pg/ml-5000pg/ml
• Sensitivity: < 5 pg/ml
• Crossreactivity:There is no detectable cross-reactivity with other relevant proteins.
• Uniprot ID:P01589
• Entrez Gene ID:3559
• Kit Components:96-well plate pre-coated with anti-CD25/IL-2sR alpha, HumanLyophilized recombinant CD25/IL-2sR alpha, Human standard, 10ng/tube, 2EaUSB Cat No Kit Component143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)143697 Sample diluent buffer, 30ml143698 Antibody diluent buffer, 12ml143699 ABC diluent buffer, 12ml143700 TMB color developing agent, 10ml143701 TMB stop solution, 10ml
• Storage and Handling:Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles.