85-1788-67　GDF-5, Recombinant, Human (BMP-14, Cartilage-Derived Morphogenetic Protein-1 (CDMP-1)) 10ug　143344

特徴

• GDF-5 is expressed in long bones during embryonic development and postnatally in articular cartilage. Mutations in the GDF-5 gene have been implicated in Grebe Syndrome, which is characterized by short stature, extra digits, short and deformed extremities, and in Hunter-Thompson type dwarfism.The mature and functional form of GDF-5 is a homodimer of two 120 amino-acid polypeptide chain (monomers) linked by a single disulfide bond. Each GDF-5 monomer is expressed as the C-terminal part of a precursor polypeptide, which also contains a 27 amino-acid signal peptide and a 354 amino-acid propeptide. This precursor undergoes intracellular dimerization, and upon secretion it is processed by a furin-type protease.Recombinant human GDF-5 is a 27.0kD homodimeric disulfide-linked protein consisting of two 120 amino acids
• License:This product is produced solely by PeproTech Inc. under a license from Biopharm GmbH, Germany.Biopharm GmbH is not responsible for the quality or fitness for use of this product.It may be used only for research purposes and may neither be used in humans nor for veterinary purposes.This product may not be used for commercial purposes.
• Biological Activity:The ED50 was determined by its ability to induce alkaline phosphatase production by ATDC-5 chondrogenic cells is 1.0-2.0ug/ml.
• Amino Acid Sequence:APLATRQGK RPSKNLKARC SRKALHVNFK APSATRQGK RPSKNLKARC SRKALHVNFK DMGWDDMGWDDWIIA PLEYEAFHCE GLCEFPLRSH LEPTNHAVIQ TLMNSMDPES TPPTCCVPTR LSPISILFID SANNVVYKQY EDMVVESCGC R
• Crossreactivity:Mouse
• Quality Control:Verified by N-terminal and Mass Spectrometry analyses (when applicable).
• Protein Content:Verified by UV Spectroscopy and/or SDS-PAGE gel.
• Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Do not vortex. For longer term storage the protein solution should be stored with a carrier protein (eg. 0.1% BSA). Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.