Specific substrate for GSK3b (Cat# G8170-46). Synthetic peptide (YRRAAVPPSPSLSRHSSPHQ(pS)EDEEE). Labeled with biotin at the N-terminus.
Peptide Sequence:Synthetic peptide (YRRAAVPPSPSLSRHSSPHQ(pS)EDEEE). Labeled with biotin at the N-terminus.
Applications:Suitable for use in Protein Kinase Assay. Other applications not tested.
Recommended Dilution:Protein Kinase Assay: 40ng of GSK3b, active to phosphorylate the biotinylated phospho-glycogen synthase peptide. Optimal dilutions to be determined by researcher.
Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
