85-1785-99　Insulin, Recombinant, Mouse (INS) 10ug　I7660-07

特徴

• E. coli-expressed, N-terminal His-tagged recombinant protein corresponding to Phe25-Ser54 of mouse Insulin-B linked by polypeptide linker (GGGGS) to Gly90-Asn110 of mouse Insulin A (UniProt accession #P01326).
• Amino Acid Sequence:FVKQHL CGSHLVEALY LVCGERGFFY TPMSGGGGSG IVDQCCTSIC SLYQLENYCN
• Preditcted Molecular Weight:10.3
• Accurate Molecular Weight:10kD (SDS-PAGE reducing conditions)
• Predicted Isoelectric Point:7.1
• Subcellular Location:Secreted
• Tissue Specificity:Pancreas
• Applications:Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested.
• Recommended Dilutions:Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -70°C. Stable for 6 months after receipt. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
• Note:Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
• Included Protein Molecular Weight Marker:168631: Unstained Protein Molecular Weight Marker, 10-70kDSuppled as a liquid in 62.5mM Tris-H3PO4, pH 7.5, 1mM EDTA, 2% SDS, 100mM DTT, 1mM sodium azide, 0.01% bromo-phenol blue, 33% glycerol. Ready to use; no need to heat, dilute or add reducing agents before use.
• Protein Bands: 10kD, 14kD, 18kD, 22kD, 26kD, 33kD, 44kD and 70kD
• Double Intesity Bands: The 26kD, 18kD and 10kD bands are at double intensity to make location and size approximation of proteins easier.
• Usage: Allow the marker to reach room temperature and mix thoroughly before use to ensure that any solids that may have precipitated at -20°C will go back into solution. Do not boil! Load the following volumes on SDS-PAGE gel:Mini gels: 3-5ul/wellLarge gel: 7ul/wellThe loading volumes listed above are recommended for gels with thickness of 0.75-1mm. The loading volume should be doubled for 1.5mm thick gels
• Store at -20°C Stable for 6 months after receipt.