HRSP12, also called ribonuclease UK114, is an endoribonuclease found predominantly in human adult kidney and liver, and which is responsible for inhibiting translation by cleaving mRNA. This protein cleaves phosphodiester bonds only in single-stranded RNA. It may be an important biomarker for heptatic carcinoma. Recombinant human HRSP12 protein, fused to His-tag at N-terminal domain, was expressed in E.coli and purified by using conventional chromatography.
Source:Recombinant protein corresponding to aa1-137 from human HRSP12, fused to His-tag at N-terminal domain, expressed in E.coli.
Molecular Weight:~16.6kD (MALDI-TOF)
Applications:Suitable for use in SDS-PAGE. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
AA Sequence:MGSSHHHHHHSSGLVPRGSHMSSLIRRVISTAKAPGAIGPYSQAVLVDRTIYISGQIGMDPSSGQLVSGGVAEEAKQALKNMGEILKAAGCDFTNVVKTTVLLADINDFNTVNEIYKQYFKSNFPARAAYQVAALPKGSRIEIEAVAIQGPLTTASL
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Source Antigen：Recombinant, E.coli
Grade：Highly Purified
Purity：~95% (SDS-PAGE)
Form：Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.1M sodium chloride, 1mM DTT, 10% glycerol.
