IL-17A/F is a cystine-linked heterodimer of IL-17A and IL-17F (1,2). IL-17A/F is produced by Th17 cells, and y8 T cells (1,2). IL-17A/F stimulates the production of pro-inflammatory cytokines and neutrophil chemoattractants, thereby functioning as a bridge between adaptive and innate immunity (1-3). Some research studies suggest that IL-17A/F may be involved in mucosal immunity against some bacterial infections and has a putative role in some autoimmune disorders (3,4). The receptor for IL-17A/F consists of a heterodimer of IL-17RA and IL-17RC and signaling through this receptor leads to the activation of the Erk1/2 and NF-kB pathways (1).
Source:Recombinant protein corresponding to human IL-17A/F, expressed in human 293 cells.
Molecular Weight:~31.396kD
Biological Activity:Determined by its ability to induce mouse IL-6 production by 3T3 MEFs WT. The ED50 of each lot is 50-200ng/ml.
Endotoxin:<0.01ng/1ug
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：10ug
Source Antigen：Recombinant, 293cells
Grade：Highly Purified
Purity：~98% (SDS-PAGE)
Form：Supplied as a lyophilized powder in PBS, pH 7.2, 20ug BSA/ug recombinant IL-17A/F. Reconstitute in sterile PBS.
