Gö6976 is an ATP-competitive PKC inhibitor specific for Ca2+-dependent PKCalpha (IC50= 2.3nM) and PKCBeta1 (IC50 = 6.2nM) isozymes (1). It is also a potent inhibitor of PKD (IC50= 20nM) (2). Researchers have demonstrated that Gö6976 blocks JNK activation (3-5) and inhibits PKCalpha-mediated, TPA-stimulated phosphorylation of CREB at Ser133 (6,7). Gö6976 is also an effective inhibitor of the tyrosine kinases Jak2 and FLT3 (8), as well as TrkA and TrkB (9).
Usage:For a 1mM stock, reconstitute the 500ug in 1.32ml DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used as a pretreatment at 0.1-10uM for 0.5-1 hr prior to treating with a stimulator or is used alone with varying treatment times lasting up to 24 hr. Soluble in DMSO at 25mg/ml; very poorly soluble in ethanol and water with maximum solubility in water at ~1-5uM.
Purity:≥99%
Form:Supplied as a lyophilized powder.
Storage and Stability:Store at -20°C under inert atmosphere. Caution: Light-sensitive. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
