USP8 is a ubiquitin specific protease that plays an important regulatory role at the level of protein turnover by preventing degradation. USP8 is involved in cell proliferation, and probably regulates the stability of STAM2 and RASGRF1. USP8 may regulate T-cell anergy mediated by RNF128 via the formation of a complex containing RNF128 and STAM2. As revealed by structur/function studies, USP8 forms a ternary complex with RNF128 and OTUB1, and interacts with the SH3 domain of STAM2 and RASGRF1. Expression of USP8 is induced Upon growth stimulation in starved human fibroblasts, and expression decreases in response to growth arrest induced by cell-cell contact.
Source:Recombinant corresponding to aa2-1118 from human USP8, fused to FLAG-tag at N-terminal, expressedin Sf9 insect cells via a baculovirus expression system.
Molecular Weight:~128kD
Applications:Suitable for for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Source Antigen：Recombinant, Sf9 insect cells
Grade：Purified
Purity：Purified (~55%)
Form：Supplied as a liquid in 45mM Tris-HCl, pH 8.0, 124mM sodium chloride, 2.4mM potassium chloride, 90ug/ml Flag peptide, 10% glycerol, 3mM DTT.
